Eun-Soo Han   -B.A. Ewha Womans University -M.S., Louisiana State University -Ph.D., Louisiana State University Office: N219 Oliphant Hall Phone: (918) 631-2310 Email: eun-han@utulsa.edu Research Interests: My research goal is to probe the biological mechanisms underlying aging and calorie restriction (CR).  I am attempting to employ novel molecular biological approaches (gene expression arrays) that seem likely to undercover altered pattern of gene expression in mice and rats under CR.  Finding differences in gene expression between ad libitum fed and CR animals may give clues to the question, “how does aging happen?”  The answer to this question is my ultimate research goal.  I have been awarded two grants, a National Institute of Health grant (1 KO1 AG00746) to study genes to acutely responsive to CR and a National Institute of Health grant (SG 5 PO1 AG14674) to study altered gene expression in transgenic mice which mimic the CR traits. Selected Publications: Gokhale, D.V., E.S. Han, V.R. Srinivasan, and D.N. Doebagkar 1984  Transfer of DNA coding for cellulase from Cellulomonas species to Bacillus subtilis by protoplost fusion.  Biotechnology Letters 6(10):627-632. Han, E.S., W.H. McGaughey, D.E. Johnson, P. Dunn, and A.I. Aronson 1988 Characterization of Bacillus thuringiensis isolates effective on resistant indian meal moth.  In:  Ganesan, A.T. and J.A. Hoch (eds.), Genetics and Biotechnology of Bacilli, Vol. 2, Academic Press, San Diego, pp. 233-238. Sakaguchi, A.Y., P.A. Lalley, G. Ghosh-Choudhury, L. Martinez, E.S. Han, A.M. Killary, S.L. Naylor, and L.-M. Wang 1989  Mouse melanoma growth stimulatory activity gene (mgsa) is polymorphic and syntenic with the W. Patch, rumpwhite, and recessive spotting loci on chromosome 5.  Genomic 5:629-632. Aronson, A.I., E.S. Han, W. McGaughey, and D. Johnson 1991  The solubility of inclusion proteins from Bacillus thuringiensis is dependent upon protoxin composition and is a factor in toxicity to insects.  Applied Environmental Microbiology 57(4):981-986. Han, E.S., M.P. Moyer, S. Naylor, and A.Y. Sakaguchi 1991 Mutation in the TP53 gene in colorectal carcinoma detected by polymerase chain reaction.  Genes, Chromosomes and Cancer 3:313-317. Sakaguchi, A.Y., V.L. Sylvia, L. Martinez, E.A. Smith, E.S. Han, P.A. Lalley, T.B. Shows, and G.G.Choudhury 1992  Assignment of tyrosine-specific T-cell phosphatase to conserved syntenic groups on human chromsome 18 and mouse chromosome 18.  Genomic 12:151-154. Han, E.S., N. Levin, N. Bengani, J. Roberts, Y. Suh, K. Karelus, and J.F. Nelson 1995  Hyperadreno-corticism and food restriction-induced life-extension in the rat:  Evidence for divergent regulation of pituitary proopiomelanocortin RNA and adrenocorticotropic hormone biosynthesis.  J. Gerontol. Biol. Sci. 50A(5):B288-B294. Han, E.S., D.H. Lu, and J.F. Nelson 1998  Food restriction differentially affects on messenger RNAs encoding the major anterior pituitary tropic hormones. J. Gerontol. Biol. Sci. 53A(5):B322-B329. Han, E.S., T.R. Evans, and J.F. Nelson 1998  Adrenocortical responsiveness to adrenocorticotropic hormone is enhanced in chronically food restricted rats. J. Nutr. 128:1415-1420.  Han, E.S., S.G. Hilsenbeck, A. Richardson, and J.F. Nelson 2000  cDNA expressin arrays reveal incomplete reversial of age-related changes in gene expression by calorie restriction. Mech. Age. Dev. 115(3):157-174. Han, E-S., H. Van Remmen, M. Steinhelper, M.A. Pahlavani, J.R. Strong, and A. Richardson 2001  Effect of age on gene expression.  In Handbook of Biology of Aging (Masoro, E.A., J. Campisi, and S. Austad, eds), Academic Press, San Diego, pp. 140-178. Han, E.S., and S.G. Hilsenbeck 2001  Array-based gene expression profiling to studying aging. Mech. Age. Dev. 122(10): 999-1018. Han, E.S., T.R. Evans, J.H. Shu, S. Lee, and J.F. Nelson 2001  Food restriction enhances endogenous and corticotropin-induced plasma elevations of free but not total corticosterone throughout life in rats. J. Gerontol. Biol. Sci. 56A(9):B391-B397. Han, E.S., T.R. Evans, S. Lee, and J.F. Nelson 2001  Food restriction differentially affects rat pituitary hormone mRNAs throughout the adult life span of male F344 rats. J. Nutr. 131:1687-1693. ,Han, E. S., Y. Wu, R. McCarter, J.F. Nelson, A. Richardson, and S.G. Hilsenbeck 2004 Reproducibility, sources of variability, pooling, and sample size: Important considerations for the design of high density oligonucleotide array experiments. J. Gerontol. Biol. Sci. 59A (4); 306-315. Fu, C., L. Xi, Y. Wu, R. McCarter, A. Richardson, M. Hickey, and E.S. Han 2004 Hepatic genes altered in expression by food restriction are not influenced by the low plasma glucose level in young male GLUT4 transgenic mice. J. Nutr. 134: 2965-2974. Cook, P., C. Fu, M. Hickey, E.S. Han, and K. S. Miller 2004 SAS programs for real-time QRT-PCR experiments having multiple independent samples. Biotechniques. 37(6): 990-995. Han, E.S and M. Hickey 2005 Microarray evaluation of dietary restriction. J. Nutr. 135: 1343-1346. Fu, C., L. Xi, R. McCarter, M. Hickey, and E.S. Han 2006 Early hypothalamic response to age-dependent gene expression by calorie restriction. Neurobiol. of Aging. 27(9): 1315-1325. Fu, C., M. Hickey, M. Morrison, R. McCarter, E.S. Han 2006 Tissue specific and non-specific changes in gene expression by aging and by early stage CR. Mech. Age. Dev. 127(12): 905-916. Research Students and Projects: Anti-aging mechanisms of calorie restriction Identifying altered gene expression by oxidative stress Teaching Fields Biol 1003-Genetics and Human Diversity Biol 4263, 6263-Selected Topics in Molecular Genetics Biol 4344, 6444-Advanced Molecular Biology Biol 4340L, 6440L- Advanced Molecular Biology Laboratory Biol 7263-Seminar in Cell/Molecular Biology Other Responsibility: Microarray Core Facility (http://www.utulsa.edu/microarray/)